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Glucose Hexokinase


(Assays)

Catalog #: GLU600

METHOD

Hexokinase/G6PD, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Linearity: 0.6-600 mg/dL
Expected Values: 70-105 mg/dL
Storage Temperature: 2-8°C
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Intended Use

For the in vitro quantitative measurement of glucose in serum.

Principle

Glucose is phosphorylated by hexokinase (HK) in the presence of adenosine triphosphate (ATP) and magnesium to form glucose-6-phosphate (G-6-P) and adenosine diphosphate (ADP). G-6-P is then oxidized by glucose-6-phosphate dehydrogenase (G-6-PDH) in the presence of nicotinamide adenine dinucleotide (NAD) producing 6-phosphogluconate and NADH. The formation of NADH causes increase in absorbance at 340 nm which is directly proportional the concentration of glucose in the sample.