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Alkaline Phosphatase


(Assays)

Catalog #: ALP480

METHOD

p-NPP, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 412 nm
Linearity: 2-1000 U/L
Expected Values: Adults 35-123 U/L, Children have a higher normal value
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of alkaline phosphatase in human serum using the Mindray BS-480 analyzer. In vitro diagnostic medical device.

Principle

ρ-Nitrophenyl phosphate is hydrolyzed to ρ-nitrophenol and inorganic phosphate. The rate at which the p-NPP is hydrolyzed, measured at 405 nm, is directly proportional to the alkaline phosphatase activity.