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Triglyceride


(Assays)

Catalog #: TRI600

METHOD

Enzymatic/GPO Trinder, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 500nm
Linearity: 1000 mg/dL
Expected Values: 44-148 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the in vitro quantitative determination of Triglycerides in serum or plasma using the Mindray BS-200 analyzer.

Principle

Serum triglycerides are hydrolyzed to glycerol and free fatty acids by lipase. In the presence of ATP and glycerol kinase (GK), the glycerol is converted to glycerol-1-phosphate. The glycerol-1-phosphate is then oxidized by glycerol phosphate oxidase (GPO) to yield hydrogen peroxide. The condensation of hydrogen peroxide with 4-chlorophenol and 4-aminophenazone (4-AA) in the presence of peroxidase (POD) produces a red colored quinonimine dye which absorbs at, or near 500nm. The intensity of the colored complex formed is directly proportional to the triglycerides concentration of the sample.