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autoLDL™ Cholesterol


(Assays)

Catalog #: LDL480

METHOD

Direct/Automated

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 546 nm
Linearity: 0-650 mg/dL
Expected Values: Desirable: <130 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
  • Description
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Intended Use

For the direct quantitative determination of low density lipoprotein cholesterol (LDL-C) in human serum or plasma using the Mindray BS-480 analyzer. For in vitro diagnostic use only.

Principle

The autoLDL™ Cholesterol Reagent is a two-part, liquid stable method for directly measuring LDL-C levels in serum or plasma. The method depends on the properties of a unique detergent which eliminates the need for any off-line pre-treatment or centrifugation steps. This detergent (Reagent 1) solubilizes only the non-LDL lipoprotein particles. The cholesterol released is consumed by cholesterol esterase and cholesterol oxidase in a non-color forming reaction. A second detergent (Reagent 2) solubilizes the remaining LDL particles and a chromogenic coupler allows for color formation. The enzyme reaction with LDL-C in the presence of the coupler produces color that is proportional to the amount of LDL cholesterol present in the sample.