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Lactate Dehydrogenase


(Assays)

Catalog #: LDH600

METHOD

Modified Wacker/Tris, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 1000 U/L
Expected Values: Male: 80-285 U/L Female: 103-227 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the in vitro quantitative kinetic determination of lactate dehydrogenase activity in serum using the Mindray BS-200 analyzer.

Principle

Lactate dehydrogenase catalyzes the oxidation of lactate to pyruvate with simultaneous reduction of NAD to NADH. The rate of NAD reduction can be measured as an increase in absorbance at 340nm. This rate is directly proportional to LD activity in serum.