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Lipase


(Assays)

Catalog #: LIP600

METHOD

Enzymatic/Colorimetric, Initial Rate

TECHNICAL INFORMATION

Format: Dry Powder
Wavelength: 550 nm
Linearity: 0-600 U/L
Expected Values: 0-62 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: 4 days RT, 21 days 2-8°C
  • Description
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Intended Use

For the in vitro quantitative kinetic determination of Pancreatic Lipase in serum using the Mindray BS-200 analyzer.

Principle

Serum pancreatic lipase acts on a natural type of substrate, 1,2-diglyceride to liberate 2-monoglyceride. The 2-monoglyceride is hydrolyzed by monoglyceride lipase (MGLP) to produce glycerol and fatty acid. Glycerol kinase (GK) then acts on the glycerol to produce glycerol-3-phosphate which is converted to dihydroxyacetone phosphate and hydrogen peroxide in a reaction catayzed by glycerol-3-phosphate oxidase (GPO). The hydrogen peroxide then reacts with 4-aminoantipyrine and N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine sodium salt (TOOS) in a reaction catalyzed by peroxidase (POD) to yield a quinone dye. The rate of increase in absorbance at 550nm is directly proportional to the Lipase activity of the sample.