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Iron - Total


(Assays)

Catalog #: IRO480

METHOD

Ferrozine, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 546 nm
Linearity: 2-500 μg/dL
Expected Values: 60-150 μg/dL
Storage Temperature: 2-8° C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of iron in serum using the Mindray BS-480 analyzer. For in vitro diagnostic use only.

Principle

Serum Iron: Transferrin-bound iron is released at an acid pH and reduced from ferric to ferrous ions. These ions react with ferrozine to form a violet colored complex which is measured spectrophotometrically at 560nm. The absorbance measured at this wavelength is proportional to serum iron concentration.