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Cholesterol


(Assays)

Catalog #: CHO480

METHOD

Enzymatic, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 505 nm
Linearity: 500 mg/dL
Expected Values: Desirable Cholesterol: <200 mg/dL, Borderline-High Cholesterol: 200-239 mg/dL, High Cholesterol: >240 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of Total Cholesterol in serum using the Mindray BS-480 analyzer.

Principle

Cholesterol esters in the sample are hydrolyzed by cholesterol esterase to release free cholesterol. The free cholesterol is oxidized by cholesterol oxidase to release hydrogen peroxide. Phenol + 4-AAP + hydrogen peroxide, in the presence of peroxidase produces a quinoneimine dye that is measured at 500nm. The absorbance at 500nm is proportional to the concentration of cholesterol in the sample.