For the quantitative determination of urea nitrogen in serum using the Mindray BS-480 analyzer. For in vitro diagnostic use only.
Urea is hydrolyzed by urease to produce ammonia and carbon dioxide. The liberated ammonia reacts with α-ketoglutarate in the presence of NADH to yield glutamate. An equimolar quantity of NADH undergoes oxidation during the reaction resulting in a decrease in absorbance that is directly proportional to the urea nitrogen concentration in the sample.