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BUN (Urea Nitrogen)


(Assays)

Catalog #: BUN600

METHOD

Urease/GLDH, Initial Rate

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 0-150 mg/dL
Expected Values: 7-18 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of urea nitrogen in serum using the Mindray BS-200 analyzer.

Principle

Urea is hydrolyzed by urease to produce ammonia and carbon dioxide. The liberated ammonia reacts with α-ketoglutarate in the presence of NADH to yield glutamate. An equimolar quantity of NADH undergoes oxidation during the reaction resulting in a decrease in absorbance that is directly proportional to the urea nitrogen concentration in the sample.