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Bilirubin - Direct


(Assays)

Catalog #: DIB600

METHOD

Diazo, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 546 nm
Linearity: 10 mg/dL
Expected Values: 0-0.5 mg/dL
Storage Temperature: RT
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of direct bilirubin in serum using the Mindray BS-200 analyzer.

Principle

Direct Bilirubin is coupled with a diazonium salt (DPD) in a strongly acid medium (pH 1 – 2). The intensity of the color of the azobilirubin produced is proportional to the Direct Bilirubin concentration and can be measured photometrically.