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Amylase


(Assays)

Catalog #: AMY480

METHOD

CNPG3, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 405 nm
Linearity: 1-2,000 U/L
Expected Values: 25-125 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative kinetic determination of α-amylase activity in human serum using the Mindray BS-480 analyzer.

Principle

α-Amylase hydrolyzes the 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNPG3) to release 2-chloro-nitrophenol and form 2-chloro-p-nitrophenyl-α-D-maltoside (CNPG2), maltotriose (G3) and glucose (G). The rate of increase in absorbance is measured at 405 nm and is proportional to the α-amylase activity in the sample.(G). The rate of increase in absorbance is measured at 405 nm and is proportional to the α-amylase activity in the sample.