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AST


(Assays)

Catalog #: AST480

METHOD

Modified IFCC, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 3-500 U/L
Expected Values: 5-34 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of Aspartate Aminotransferase (AST) in human serum using the Mindray BS-480 analyzer.

Principle

Aspartate aminotransferase (AST) catalyzes the transfer of the amino group from L-aspartate to α-Ketoglutarate to yield oxalacetate and L-glutamate. The oxalacetate undergoes reduction with simultaneous oxidation of NADH to NAD in the malate dehydrogenase (MDH) catalyzed indicator reaction. The resulting rate of decrease in absorbance at 340nm is directly proportional to the AST activity. Lactate dehydrogenase (LDH) is added to prevent interference from endogenous pyruvate which is normally present in serum.