The DRI® Propoxyphene Assay is intended for qualitative and semi-quantitative determination of propoxyphene in human urine.
The DRI Propoxyphene Assay is a homogeneous enzyme immunoassay using ready-to-use liquid reagents. The assay uses specific antibodies, which can detect propoxyphene in urine. The assay is based on the competition of an enzyme glucose-6-phosphate dehydrogenase (G6PDH) labeled drug and the drug from the urine sample for a fixed amount of specific antibody binding sites. In the absence of drug from the sample, the specific antibody binds the drug labeled G6PDH and the enzyme activity is inhibited. This phenomenon creates a relationship between drug concentration in urine and the enzyme activity. The enzyme G6PDH activity is determined spectrophotometrically at 340 nm by measuring its ability to convert nicotinamide adenine dinucleotide (NAD) to NADH.