The DRI® Methaqualone Assay is intended for the qualitative and semi-quantitative determination of methaqualone in human urine.
The DRI Methaqualone Assay is a homogeneous enzyme immunoassay using ready-to-use liquid reagents. The assay uses specific antibody, which can detect methaqualone in urine. The assay is based on the competition of a drug labeled with enzyme, glucose 6-phosphate dehydrogenase (G6PDH), and the free drug from the urine sample for a fixed amount of specific antibody binding sites. In the absence of free drug from the sample, the specific antibody binds the drug labeled with G6PDH and the enzyme activity is inhibited. This phenomenon creates a direct relationship between the drug concentration in urine and the enzyme activity. The G6PDH activity is determined spectrophotometrically at 340 nm by measuring its ability to convert nicotinamide adenine dinucleotide (NAD) to NADH.