The CEDIA Heroin Metabolite (6-Acetylmorphine, or 6-AM) Assay is a homogeneous enzyme immunoassay for the in vitro qualitative determination of heroin metabolite (6-AM) in human urine on automated clinical chemistry analyzers. Measurements are used as an aid in the detection of heroin use or overdose.
In the CEDIA Heroin Metabolite Assay, the sample competes with 6-AM conjugated to enzyme donor for antibody binding sites. If 6-AM is present in the sample, it binds to antibody, leaving the ED-6-AM conjugate free to reassociate with enzyme acceptor to form active -galactosidase. If no 6-AM is present in the sample, antibody binds to the ED-6-AM conjugate, inhibiting the reassociation of inactive β-galactosidase fragments, and thus reducing the amount of active enzyme formed. The amount of active enzyme formed and resultant absorbance change are proportional to the amount of 6-AM present in the sample.