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Homocysteine


(Assays)

Catalog #: HOM480

METHOD

Enzymatic, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 1-46 µmol/L
Expected Values: 5-15 µmol/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
  • Description
  • Ordering Information
  • Related Products
  • Documentation

Intended Use

The Liquid Stable 2-Part Homocysteine Reagent is intended for in vitro quantitative determination of total homocysteine in human serum and plasma on the Mindray BS-480 analyzer. The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.

Principle

Bound or dimerized homocysteine (oxidized form) is reduced to free homocysteine, which then reacts with serine catalyzed by cystathionine beta-synthase (CBS) to form cystathionine. Cystathionine in turn is broken down by cystathionine beta-lyase (CBL) to form homocysteine, pyruvate and ammonia. Pyruvate is then converted by lactate dehydrogenase (LDH) to lactate with nicotinamide adenine dinucleotide (NADH) as coenzyme. The rate of NADH conversion to NAD+ is directly proportional to the concentration of homocysteine (∆ A340 nm).