Enzymatic assay for the quantitative in vitro determination of glycated protein (fructosamine) in human serum or plasma.
Reagent 1 contains Proteinase K which digests the glycated protein to yield glycated protein fragments. Ketoamine oxidase (KAO) in reagent 2 oxidizes the ketoamine bond of the glycated protein fragments. As a result hydrogen peroxide is released and it is involved in a colorimetric Trinder end-point reaction. The amount of color developed at 550 nm is proportional to the concentration of glycated protein in the sample.