Plasma fibrinogen assay kit is an in-vitro immunoturbidimetric test for quantifying the amount of native, intact, immunoreactive fibrinogen present in human blood plasma. (Calibrators and Controls Included)
The plasma specimen (calibrator, control or patient) is first allowed to incubate at 37°C with Reagent I (a buffered solution of PEG and other immunocomplex enhancers to obtain a reference absorbance blank at 340 nm - 700 nm with a bichromatic analyzer, which minimizes the interference(s) due to bilirubin, hemoglobin, lipoproteins, or chylomicrons. Addition of Reagent II (anti-human fibrinogen in buffer) results in the formation of an insoluble fibrinogen immunocomplex whose absorbance is measured at 340 nm after three minutes. The blank absorbance reading is subtracted from the test absorbance and compared to a calibrator to obtain the fibrinogen concentration in the plasma specimen.