The DRI® Cotinine Assay is an in vitro diagnostic medical device intended for the qualitative and semi-quantitative determination of Cotinine in human urine at a cutoff level of 500 ng/mL. This assay is intended as an aid in the detection of cotinine after use or exposure to tobacco products.
The DRI Cotinine Assay is a liquid, ready-to-use homogeneous enzyme immunoassay. The assay is based on competition between continine labeled with glucose-6-phosphate dehydrogenase (G6PDH) enzyme and free cotinine in the sample for a fixed amount of cotinine-specific antibody binding sites. The enzyme G6PDH activity is determined spectrophotometrically at 340 nm by measuring its ability to convert nicotinamide adenine dinucleotide (NAD) to NADH.