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Cotinine


(Assays)

METHOD

DRI Urine Drug Screening

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 2000 ng/mL
Storage Temperature: 2 - 8° C
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Intended Use

The DRI® Cotinine Assay is an in vitro diagnostic medical device intended for the qualitative and semi-quantitative determination of Cotinine in human urine at a cutoff level of 500 ng/mL. This assay is intended as an aid in the detection of cotinine after use or exposure to tobacco products.

Principle

The DRI Cotinine Assay is a liquid, ready-to-use homogeneous enzyme immunoassay. The assay is based on competition between continine labeled with glucose-6-phosphate dehydrogenase (G6PDH) enzyme and free cotinine in the sample for a fixed amount of cotinine-specific antibody binding sites. The enzyme G6PDH activity is determined spectrophotometrically at 340 nm by measuring its ability to convert nicotinamide adenine dinucleotide (NAD) to NADH.