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autoHDL™ Cholesterol


(Assays)

Catalog #: HDL480

METHOD

Direct/Automated

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 570 nm
Linearity: 2 - 150 mg/dL
Expected Values: Males: 30-70 mg/dL Females: 30-85 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination high-density lipoprotein cholesterol in human serum or plasma using the Mindray BS-480 analyzer. For in vitro diagnostic use only.

Principle

The Liquid autoHDL™ Cholesterol assay is a homogeneous method for directly measuring serum HDL-C levels without the need for any off-line pretreatment or centrifugation steps. The method is in a two-reagent format. The first reagent contains α-cyclodextrin and dextran sulfate to stabilize LDL, VLDL, and chylomicrons. The second reagent contains PEG modified enzymes that selectively react with the cholesterol present in the HDL particles. Consequently, only the HDL cholesterol is subject to cholesterol measurement.