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Glucose Hexokinase


(Assays)

Catalog #: GLU480

METHOD

Hexokinase/G6PD, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 0.6-600 mg/dL
Expected Values: 70-105 mg/dL
Storage Temperature: 2-8°C
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Intended Use

For the in vitro quantitative measurement of glucose in serum using the Mindray BS-480 analyzer.

Principle

Glucose is phosphorylated by hexokinase (HK) in the presence of adenosine triphosphate (ATP) and magnesium to form glucose-6-phosphate (G-6-P) and adenosine diphosphate (ADP). G-6-P is then oxidized by glucose-6-phosphate dehydrogenase (G-6-PDH) in the presence of nicotinamide adenine dinucleotide (NAD) producing 6-phosphogluconate and NADH. The formation of NADH causes increase in absorbance at 340 nm which is directly proportional the concentration of glucose in the sample.