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ALT


(Assays)

Catalog #: ALT480

METHOD

Modified IFCC, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 3-500 U/L
Expected Values: 4-36 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
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Intended Use

For the quantitative determination of Alanine Aminotransferase (ALT) in serum using the Mindray BS-480 analyzer.

Principle

ALT catalyzes the transfer of the amino group from L-alanine to α-ketoglutarate resulting in the formation of pyruvate and L-glutamate. Lactate dehydrogenase catalyzes the reduction of pyruvate and the simultaneous oxidation of NADH to NAD. The resulting rate of decrease in absorbance is directly proportional to ALT activity.